Dialysis with icodextrin interferes with measurement of serum alpha-amylase activity
نویسندگان
چکیده
منابع مشابه
Measurement of amylase activity.
The Nelson photoelectric method for the determination of glucose in biological fluids (1) has proved to be the method of choice in this laboratory and in many others, owing to its reproducibility, stability of color, and excellent agreement with the Beer-Lambert law. Nelson, however, states that the method is unsuitable for the determination of diastatic activity. Since it is convenient and eco...
متن کاملMeasurement of Amylase Activity*
The Nelson photoelectric method for the determination of glucose in biological fluids (1) has proved to be the method of choice in this laboratory and in many others, owing to its reproducibility, stability of color, and excellent agreement with the Beer-Lambert law. Nelson, however, states that the method is unsuitable for the determination of diastatic activity. Since it is convenient and eco...
متن کاملKinetic nephelometric procedure for measurement of amylase activity in serum.
We developed a kinetic procedure for determination of amylase activity in serum by use of nephelometric measurements. Light scattering from the substrate, a stable suspension of starch, is decreased as amylase hydrolyzes the starch to soluble fragments. Values obtained for serum amylase correlate closely with those determined by a method in which a starch-dye complex is used. Precision of the n...
متن کاملColorimetry of carbohydrates with m-aminophenol, and its proposed use in measurement of serum amylase activity.
In the reaction of m-aminophenol with carbohydrates in a thiourea/acetic acid reagent, addition of borate suppresses chromogen formation for hexoses and pentoses, and enhances it for disaccharides. All reaction products absorb maximally at 520 nm. The relatively greater intensity of the m-aminophenol/maltose reaction product was used in a saccharogenic method for amylase, with low blank reading...
متن کاملOptimized conditions for determining activity concentration of alpha-amylase in serum, with 1,4-alpha-D-4-nitrophenylmaltoheptaoside as substrate.
We describe a method for measuring the catalytic activity of alpha-amylase (EC 3.2.1.1) in serum and urine, by use of a defined substrate: 1,4-alpha, D-4-nitrophenyl maltoheptaoside. We use a phosphate buffer of pH 7.10, containing chloride as activator and alpha-glucosidase (EC 3.2.1.20) as the auxiliary enzyme. After a lag phase of 4 min at 25 degrees C or 30 degrees C, or 3 min at 37 degrees...
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ژورنال
عنوان ژورنال: Nephrology Dialysis Transplantation
سال: 2002
ISSN: 1460-2385
DOI: 10.1093/ndt/17.11.1988